2026
366. Discovery of the Phosphonate Flavophos Produced by Burkholderia
Simon, M.A.; Ramos-Figueroa, J.S.; Reyes Lopez, V.; Zhu, L.; Ongpipattanakul, C.; Hoffpauir, Z.A.; Lamb, A.L.; Nair, S.K.; van der Donk, W.A.* (2026). J. Am. Chem. Soc., 148, 18030-18043.
365. Structure, Biosynthesis, and Bioactivity of Nostolysamides
Weir, E.; Anterola, I.: van der Donk, W.A.* (2026). Biochemistry, 65, 1578-1591..
364. Co-Opting the Bacterial Lipoprotein Pathway in the Biosynthesis of a Lipidated Macrocyclic Peptide
Chen, J.Y.; Zhu, L.; Zhang, K.Y; Berthold, D.A.; van der Donk, W.A.* (2026). Proc. Natl. Acad. Sci. USA, e2532672123.
363.An Unusual Ring Pattern in the Rosβ Lanthipeptide of the Two-Component Lantibiotic Roseocin
Desormeaux, E.K.; Zhu, L.; Luo, Y.; Chaudhary, S.; Sareen, D.; van der Donk, W.A.* (2026). J. Nat. Prod., 89, 519-527.
362. Oxidative Peptide Backbone Cleavage by a HEXXH Enzyme During RiPP Biosynthesis
Ouyang, Y.; Yu, Y.; Zhu, L.; Nguyen, D.T.: van der Donk, W.A.* (2026). J. Am. Chem. Soc., 148, 3551-3561.
2025
361.The Biosynthetic Pathway to the Pyrroloiminoquinone Marine Natural Product Ammosamide C
Ramos Figueroa, J.; Zhu, L.; Halliman, M.; van der Donk, W.A.* (2025). J. Am. Chem. Soc., 147, 46315-46323.
360. Aminoacyl-tRNA Specificity of a Ligase Catalyzing Non-Ribosomal Peptide Extension
Nguyen, D.T.; Ramos-Figueroa, J.S.; Vinogradov, A.A.; Goto, Y.; Gadgil, M.G.; Splain, R.A.; Suga, H.; van der Donk, W.A.*; Mitchell, D.A.* (2025). J. Am. Chem. Soc., 147, 37893-37898.
359. Large Protein-Like Leader Peptides Engage Differently with RiPP Halogenases and Lanthionine Synthetases
Vidya, F.N.U.; Luo, Y.; Wu, H.; van der Donk, V.A.; McShan, A.C*; Agarwal, V.* (2025). Nat. Comm., 16, 9273.
358. Characterization of S-Glycosylated Glycocins Containing Three Disulfides
Martini, R.M.; Padhi, C.; van der Donk, W.A.* (2025). J. Ind. Microbiol. Biotechnol., 52, 1367-5435.
357. Combatting Virulent Gut Bacteria by Inhibiting the Biosynthesis of a Two-component Lanthipeptide Toxin
Moreira, R.; Chakraborty, B.; Yang, Y.; Padhi, C.; 1, Satish K. Nair, S.K.*; van der Donk, W.A.* (2025). Nat. Comm., 16, 6936.
356. Biosynthesis of Biphenomycinlike Macrocyclic Peptides by Formation and Cross-Linking of Ortho-Tyrosines
Padhi, C.; Zhu, L.; Chen J.Y.; Moreira, R.; van der Donk, W.A.* (2025). J. Am. Chem. Soc., 147, 23781-23796.
355. A New Heme Enzyme Family Forms Hydrazine Groups in Diverse Biosynthetic Pathways
Kenney, G.E.; Wang, K-K.A.; Ng, T.L.; van der Donk, W.A.; Balskus, E.P.* (2025). bioRxiv. DOI: 10.1101/2025.05.19.655019.
354. Genome Mining for Natural Products Made by Multinuclear Iron-dependent Oxidation Enzymes (MNIOs)
Yu, Y.; van der Donk, W.A.* (2025). Methods in Enzymology, 717, 89-117.
353. Substrate Recognition by a Peptideaminoacyl-tRNA Ligase
Ramos Figueroa, J.; Liang, H.; van der Donk, W.A.* (2025). Proc. Natl. Acad. Sci. USA, 122, e2423858122.
352. Transformation-Guided Genome Mining Provides Access to Brominated Lanthipeptides
Saha, N.; Vidya, F.N.U.; Luo, Y.; van der Donk, W.A.; Agarwal, V.* (2025). Organic Letters, 27, 984-988.
2024
351. Kinetic Analysis of Cyclization by the Substrate-Tolerant Lanthipeptide Synthetase ProcM
Desormeaux, E.K.; Barksdale, G.; van der Donk, W.A.*(2024). ACS Catalysis, 14, 18310-18321.
350. Substrate Specificity of a Methyltransferase Involved in the Biosynthesis of the Lantibiotic Cacaoidin
Liang, H.; Luo, Y.; van der Donk, W.A.* (2024). Biochemistry, 63, 2493-2505.
349. Bibacillin 1: a two-component lantibiotic from Bacillus thuringiensis
Moreira, R.; Yang, Y.; Luo, Y.; Gilmore, M.S.; van der Donk, W.A.* (2024). RSC Chem Biol., 5, 1060-1073.
348. Initial Characterization of the Viridisins’ Biological Properties
Vermeulen, R.*; Du Preez van Staden, A.; Ollewagen, T.; van Zyl, L.J.; Luo, Y.; van der Donk, W.A.; Dicks, L.M.T.; Smith, C.; Trindade, M. (2024). ACS Omega, 9, 31832-31841.
347. Use of a head-to-tail peptide cyclase to prepare hybrid RiPPs
Le, T.; Zhang, D.; Martini, R.M.; Biswas, S.; van der Donk, W.A.*(2024). Chem. Comm., 60, 6508-6511.
346. Multinuclear Non-Heme Iron Dependent Oxidative Enzymes (MNIOs) Involved in Unusual Peptide Modifications
Chen, J.Y.; van der Donk, W.A.* (2024). Curr. Opin. Chem. Biol., 80, 102467.
345. Unexpected Transformations during Pyrroloiminoquinone Biosynthesis
Ramos Figueroa, J.; Zhu, L.; van der Donk, W.A.* (2024). J. Am. Chem. Soc., 146, 14235-14245.
344. Biosynthesis of Macrocyclic Peptides with C-Terminal β-Amino-α-keto Acid Groups by Three Different Metalloenzymes
Nguyen, D.T.; Zhu, L.; Gray, D.L.; Woods, T.J.; Padhi, C.; Flatt, K.M.; Mitchell, D.A.*; van der Donk, W.A.* (2024). ACS Cent. Sci., 10, 1022-1032.
343. Genome Mining for New Enzyme Chemistry
Nguyen, D.; Mitchell, D.A.*; van der Donk, W.A.* (2024). ACS Catal., 14, 4536-4553.
342. Activity of Gut-Derived Nisin-like Lantibiotics against Human Gut Pathogens and Commensals
Zhang, Z.J.*; Wu, C.; Moreira, R.; Dorantes, D.; Pappas, T.; Sundararajan, A.; Lin, H.; Pamer, E.G*; van der Donk, W.A. (2024). ACS Chem. Biol., 19, 357–369.
341. Facile Method for Determining Lanthipeptide Stereochemistry
Luo, Y.; Xu, S.; Frerk, A.; van der Donk, W.A.* (2024). Anal. Chem., 96, 1767–1773.
340. PEARL-Catalyzed Peptide Bond Formation after Chain Reversal by Ureido-Forming Condensation Domains
Yu, Y.; van der Donk, W.A.* (2024). ACS Cent. Sci., 10, 1242-1250.
339. Expression and Subcellular Localization of Lanthipeptides in Human Cells
Eslami, S.M.; Padhi, C.; Rahman, I.R.; van der Donk, W.A.* (2024). ACS Synth. Biol., 13, 2128-2140.
338. Proteases Involved in Leader Peptide Removal during RiPP Biosynthesis
Eslami, S.; van der Donk, W.A.* (2024). ACS Bio Med Chem Au, 4: 20-36.
2023
337. Investigation into the Mechanism of Action of the Antimicrobial Peptide Epilancin 15X
Wu, C.; Lower, B.A.; Moreira, R.; Dorantes, D.; Le, T.; Giurgiu, C.; Shi, Y.; van der Donk, W.A.* (2023). Front. Microbiol., 14: 1247222.
336. Structures of the Holoenzyme TglHI Required for 3-Thiaglutamate Biosynthesis
Zheng, Y.; Xu, X.; Fu, X.; Zhou, X.; Dou, C.; Yu, Y.; Yan, W.; Yang, J.; Xiao, M.; van der Donk, W.A.; Zhu, X.; Cheng, W.* (2023). Structure, 31, 1220–1232.
335. The Circular Bacteriocin Enterocin NKR-5-3B Has an Improved Stability Profile Over Nisin
Wang, C.K.; Huang, Y.-H.; Shabbir, F.; Pham, H.T.; Nicole Lawrence, N.; Benfield, A.H.; van der Donk, W.A.; Henriques, S.T.; Turner, M.S.; David J Craik, D.J.* (2023). Peptides, 167, 171049.
334. Macrocyclization and Backbone Rearrangement During RiPP Biosynthesis by a SAM-Dependent Domain-of-Unknown-Function 692
Ayikpoe, R.S; Zhu, L.; Chen, J.Y.; Ting, C.P.; van der Donk, W.A.* (2023). ACS Cent. Sci., 9, 1008-1018.
333. Sequence Controlled Secondary Structure Is Important for the Site-selectivity of Lanthipeptide Cyclization
Mi, X.; Desormeaux, E.K.; Le, T.L.; van der Donk, W.A.*; Shukla, D.* (2023). Chem. Sci., 14, 6904-6914.
332. A Novel Pathway for Biosynthesis of the Herbicidal Phosphonate Natural Product Phosphonothrixin Is Widespread in Actinobacteria
Bown, L.; Hirota, R.; Goettge, M.; Cui, J.; Krist, D.; Zhu, L.; Giurgiu, C.; van der Donk, W.A.; Ju, K.-S.; Metcalf, W.W.* (2023). J. Bacteriol., 205, e00485-22.
331. Synthesis of Fluorescent Lanthipeptide Cytolysin S Analogs by Late-Stage Sulfamidate Ring-Opening
Mazo, N.; Rahman, I.R.; Navo, C.D.; Peregrina, J.M.; Busto, J.H.; van der Donk, W.A.; Jiménez-Osés, G.* (2023) Org. Lett., 25, 1431-1435.
330. Improved production of class I lanthipeptides in Escherichia coli
Lee, H.; Wu, C.; Desormeaux, E.K.; Sarksian, R.; van der Donk, W.A.* (2023) Chem. Sci., 14, 2537-2546.
329. The Mechanism of thia-Michael Addition Catalyzed by LanC Enzymes
Ongpipattanakul, C.; Liu, S.; Luo, Y.; Nair, S.K.*; van der Donk, W.A.*(2023) Proc. Natl. Acad. Sci. USA, 120, e2217523120.
328. syn-Elimination of Glutamylated Threonine in Lanthipeptide Biosynthesis
Sarksian, R.; Zhu, L.; van der Donk, W.A.* (2023) Chem. Commun., 59, 1165-1168.
2022
327. Class V Lanthipeptide Cyclase Directs the Biosynthesis of a Stapled Peptide Natural Product
Pei, Z.-F.; Zhu, L.; Sarksian, R.; van der Donk, W.A.*; Nair, S.K.* (2022) J. Am. Chem. Soc., 144, 17549-17557.
326. A Scalable Platform to Discover Antimicrobials of Ribosomal Origin
Ayikpoe, R.S; Shi, C.; Battiste, A.J.; Eslami, S.M.; Ramesh, S.; Simon, M.A.; Bothwell, I.R.; Lee, H.; Rice, A.J.; Ren, H.; Tian, Q.; Harris, L.A.; Sarksian, R.; Zhu, L.; Frerk, A.M.: Precord, T.W.; van der Donk, W.A. *; Mitchell, D.A.*; Huimin Zhao, H.* (2022) Nat. Commun., 13, 6135.
325. Mechanism of Action of Ribosomally Synthesized and Post-Translationally Modified Peptides
Ongpipattanakul, C.; Desormeaux, E.K.; DiCaprio, A.; van der Donk, W.A.*; Mitchell, D.A.*; Nair, S.K.* (2022) Chem. Rev., 122, 14722-14814.
324. Mechanistic Studies on Dehydration in Class V Lanthipeptides
Liang, H.; Lopez, I.J.; Sánchez-Hidalgo, M.; Genilloud, O.; van der Donk, W.A.* (2022) ACS Chem. Biol., 17, 2519-2527.
323. Divergent Evolution of Lanthipeptide Stereochemistry
Sarksian, R.; van der Donk, W.A.* (2022) ACS Chem. Biol., 17, 2551-2558.
322. Substrate Specificity of the Flavoenzyme BhaC1 that Converts the Indole of Trp into a Hydroxyquinone
Daniels, P.N.; van der Donk, W.A.* (2023) Biochemistry, 62, 378-387.
321. Biosynthesis of 3-Thia-α-Amino Acids on a Carrier Peptide
Yu, Y.; van der Donk, W.A.*(2022) Proc. Natl. Acad. Sci., 29, e2205285119.
320. Accessing Diverse Pyridine-based Macrocyclic Peptides by a Two-Site Recognition Pathway
Nguyen, D.T.; Le, T.T.; Rice, A.J.; Hudson, G.A.; van der Donk, W.A.*; Mitchell, D.A.* (2022) J. Am. Chem. Soc., 144, 11263-11269.
319. Macrocyclization and Backbone Modification in RiPP Biosynthesis
Lee, H.; van der Donk, W.A.* (2022) Ann. Rev. Biochem., 91, 269-294.
318. Substrate Recognition by the Peptidyl-(S)-2-Mercaptoglycine Synthase TglHI During 3-Thiaglutamate Biosynthesis
McLaughlin, M.I.; Yu, Y.; van der Donk, W.A.* (2022) ACS Chem. Biol., 17, 930-940.
317. Unexpected Methyllanthionine Stereochemistry in the Morphogenetic Lanthipeptide SapT
Sarksian, R.; Hegemann, J.D.; Simon, M.A.; Acedo, J.Z.; van der Donk, W.A.* (2022) J. Am. Chem. Soc., 144, 6373-6382.
316. Mechanism of Radical S-Adenosyl-l-methionine Adenosylation: Radical Intermediates and the Catalytic Competence of the 5'-Deoxyadenosyl Radical
Lundahl, M.N.; Sarksian, R.; Yang, H.; Jodts, R.J.; Pagnier, A.; Smith D.F.; Mosquera, M.A.; van der Donk, W.A.; Hoffman, B.M.; Broderick, W.E.; Broderick, J.B.* (2022) Chemical Science, 14(10), 2537–2546.
315. A biosynthetic pathway to aromatic amines that uses glycyl-tRNA as nitrogen donor
Daniels, P.N.; Lee, H.; Splain, R.A.; Ting, C.P.; Zhu, L.; Zhao, X.; Moore, B.S.; van der Donk, W.A.* (2022) Nature Chem., 14, 71-77.
2021
314. Structural and Mechanistic Investigations of Protein S-Glycosyltransferases
Fujinami, D.; Garcia de Gonzalo, C.; Biswas, S.; Hao, Y.; Wang, H.; Garg, N.; Lukk, T.; Nair, S.K.*; van der Donk, W.A.* (2021) Cell Chem. Biol., 28, 1740-1749.
313. Substrate Sequence Controls Regioselectivity of Lanthionine Formation by ProcM
Le, T.; Fouque, K.J.D.; Santos-Fernandez, M.; Navo, C.D.; Jiménez-Osés, G.; Sarksian, R.; Fernandez-Lima, F.*; van der Donk, W.* (2021) J. Am. Chem. Soc., 143, 18733-18743.
312. Structure-Activity Relationships of Enterococcal Cytolysin
Rahman, I.R.; Sanchez, A.: Tang, W.; van der Donk, W.A.* (2021) ACS Infect. Dis., 7, 2445-2454.
311. The Antimicrobial Activity of the Glycocin Sublancin Is Dependent on an Active Phosphoenolpyruvate-Sugar Phosphotransferase System
Biswas, S.; Wu, C.; van der Donk, W.A.* (2021) ACS Infect. Dis., 7, 2402-2412.
310. Exploring Structural Signatures of the Lanthipeptide Prochlorosin 2.8 Using Tandem Mass Spectrometry and Trapped Ion Mobility-Mass Spectrometry
Jeanne Dit Fouque, K.; Hegemann, J.; Santos-Fernandez, M.; Le, T.; Gomez-Hernandez, M.; van der Donk, W.; Fernandez-Lima, F.* (2021) Anal. Bioanal. Chem., 413, 4815-4824.
309. Peptide Backbone Modifications in Lanthipeptides
Ayikpoe, R.S.; van der Donk, W.A.* (2021) in Methods in Enzymology; Petersson, E.J., Ed.; Academic Press: Cambridge, 573-621.
308. Structural Analysis of Class I Lanthipeptides from Pedobacter lusitanus NL19 Reveals Unusual Ring Topology
Bothwell, I.R.: Caetano, T.; Sarksian, R.; Mendo, S.*; van der Donk, W.A.* (2021) ACS Chem. Biol., 16, 1019-1029.
307. Biosynthesis of Fosfomycin in Pseudomonads Reveals a New Enzymatic Activity in the Metallohydrolase Superfamily
Simon, M.A.; Ongpipattanakul, C.; Nair, S.K.*: van der Donk, W.A.* (2021) Proc. Natl. Acad. Sci. USA, 118, e2019863118.
306. Overall Retention of Methyl Stereochemistry During B12-Dependent Radical SAM Methyl Transfer in Fosfomycin Biosynthesis
McLaughlin, M.I.; Pallitsch, K.; Wallner, G.; van der Donk, W.A.*; Hammerschmidt, F.* (2021) Biochemistry, 60, 1587-1596.
305. Engineering of New-to-Nature Ribosomally Synthesized and Post-Translationally Modified Peptide Natural Products
Wu, C.; van der Donk, W.A.* (2021) Curr. Opin. Biotechnol., 69, 221-231.
304. LanCLs Add Glutathione to Dehydroamino Acids Generated at Phosphorylated Sites in the Proteome
Lai, K.-Y.; Galan, S.R.G.; Zeng, Y.; He, C.; Riedl, J.; Raj, R.; Chooi, K.P.; Garg, N.; Jones, L.H.; Hutchings, G.J.; Mohammed, S.; Nair, S.K.; Chen, J.*; Davis, B.G.*; van der Donk, W.A.* (2021) Cell, 184, 2680-2695.
303. MicroED in Natural Product and Small Molecule Research
Danelius, E.; Halaby, S.; van der Donk, W.A.*; Gonen, T.* (2021) Nat. Prod. Rep., 38, 423-431.
302. Mechanisms and Evolution of Diversity-Generating RiPP Biosynthesis
Le, T.; van der Donk, W.A.* (2021) Trends Chem., 3, 266-278.
301. New Developments in RiPP Discovery, Enzymology and Engineering
Montalbán-López, M.; Scott, T.A.; Ramesh, S.; Rahman, I.R.; van Heel, A.; Viel, J.H.; Bandarian, V.; Elke Dittmann, E.; Genilloud, O.; Goto, Y.; Grande Burgos, M.J.; Hill, C.; Kim, S.; Koehnke, J.; Latham, J.A.; Link A.J.; Martínez, B.; Nair, S.K.; Nicolet, Y.; Rebuffat, S.; Sahl, H.-G.; Sareen, D.; Schmidt, E.W.; Schmitt, L.; Severinov, K.; S Süssmuth, R.D.; Truman, A.W.; Wang, H.; Weng, J.-K.; van Wezel, G.P.; Zhang, Q.; Zhong, J.; Piel, J.;* Mitchell, D.A;* Kuipers, O.P.;* van der Donk, W.A.* (2021) Nat. Prod. Rep., 38, 130-239.
